Custom antibody formats

Mutation tools

Isotype conversion

Assay and sequence integration

IMGT alignments

Batch cloning

The system tracks all unique combinations of VH and VL sequences, which antibodies they occur in, and any squence variants generated during development.

Registration of new antibody variants, including bi-, multi-specific and ligand-fusion antibodies is made fast and errorless using Bionamic's easy to use batch cloning functionality. Customizable naming conventions are automatically applied.

The full discovery and development history of an antibody is available and accessible by simple navigation throughout the network of relations.

Simply address questions like

  • Has the VH-VL contained in a particular antibody been mutated, if so, how, when, from what and by whom?
  • Which other antibodies use this (or some variant of this) VH-VL?
  • Which productions and purifications have been made from this antibody (or any antibody containing this VH-VL or selected against similar targets) and what assay data has been collected under XYZ condition?

No fixed workflows - start from anywhere, add and connect anything.

The antibody discovery process - a trail of intertwined items

An antibody campaign is a top structure under which all antibody related data could be generated and organized.

In a campaign, libraries - or pools of antibodies - are created by registering an animal immunization or by panning a recombinant library against a target.
Individual clones from a library are isolated into library samples, and can be placed in microtiter plates.

Bionamic’s sample and plate system does not require any fixed templates or workflows, instead it provides a powerful tool to select and move any samples into new plates.
Sample assays can be uploaded using a flexible import tool where assay values are assigned to individual samples. The samples may, depending on your workflow, also have antibody sequence data associated with them.

The sample assay selection tool is a simple but powerful way to select samples based on performance across multiple types of assays.
Antibody sequences could be mapped to the individual library samples in which they are identified.

Sequences may be registered on the DNA or protein level. If DNA sequences are registered, they are automatically validated, and translated into protein sequences.

Each unique combination of variable region sequences are stored as VH-VL pairs and carefully traced across all antibodies in the system. The VH-VLs are IMGT numbered, lineage traced, and analyzed for common PTM motifs.
During development, mutations may be introduced in VH-VL sequences using Bionamic's IMGT based custom mutation schemes, called transformations.

Transformations can be applied in batch and will connect each mutated VH-VL with the full history of its parental sequences, eliminating any future confusion about the variant origin.
Bionamic’s molecular templates provide an easy and efficient way to generate new antibodies based on constant regions, VH-VLs and ligand sequences.

The templates allow batch generation of any type of antibodies construct, including multispecific or antibodies fused with other proteins. The templates work both on the protein and (optionally) DNA level and are able to generate, name and register both antibodies and associated plasmids.
Production and purification of antibodies can also be registered and tracked with fast and simple tools. The resulting batches and lots can be annotated with custom protocols for production (cell line, volume, ...) and purification (column, buffer, ...).

The system automatically identifies the combinations of plasmids (if available) that can be used to produce any antibody.
Analysis of data from functional assays and quality control of purified antibody samples can be performed using the same tools used to do hit picking of library samples during the screening phase.