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Bionamic is developed for antibody discovery
by researchers for researchers.

Features and concepts central to the Bionamic platform

The antibody discovery process

The workflow described below is a simplified example on how an antibody discovery process could look. The purpose of this example is to demonstrate how different concepts in the Bionamic platform are connected, and should not be interpreted as a built-in workflow. Instead, Bionamic’s strategy is to provide easy-to-use tools to map the real discovery and development process to our data structure.

An antibody campaign is the top structure under which all antibody related data will be generated and organized. In a campaign, libraries - or pools of antibodies - are created by registering an animal immunization or by panning a recombinant library against a target.
Individual clones from a library are isolated into library samples, and can be placed in microtiter plates. Bionamic’s sample and plate system does not require any fixed templates or workflows, instead it provides a powerful tool to select and move any samples into new plates.
Sample assays can be uploaded using a flexible import tool where assay values are assigned to individual samples. The samples may, depending on your workflow, also have antibody sequence data associated with them. The sample assay selection tool is a simple but powerful way to select samples based on performance across multiple types of assays. The tool lets the user use Bionamic’s tagging system to group samples containing antibodies with interesting binding properties. The tag can subsequently be used to identify the complete set of unique variable region sequences after sequencing.
Antibody sequences are mapped to the individual library samples in which they are identified. Sequences may be registered on the DNA or protein level. If DNA sequences are registered, they are automatically validated, transcribed to RNA and translated into protein sequences. Each unique combination of variable region sequences are stored as VH-VL pairs and carefully traced across all antibodies in the system. The VH-VLs are IMGT numbered, lineage traced, and analyzed for common PTM motifs.
During development, mutations may be introduced in VH-VL sequences using Bionamic's IMGT based custom mutation schemes, called transformations. Transformations can be applied in batch and will connect each mutated VH-VL with the full history of its parental sequences, eliminating any future confusion about the variant origin.
Bionamic’s molecular templates provide an easy and efficient way to generate new antibodies based on constant regions, VH-VLs and ligand sequences. The templates allow batch generation of any type of antibodies construct, including multispecific or antibodies fused with other proteins. The templates work both on the protein and (optionally) DNA level and are able to generate, name and register both antibodies and associated plasmids.
Production and purification of antibodies can also be registered and tracked with fast and simple tools. The resulting batches and lots can be annotated with custom protocols for production (cell line, volume, ...) and purification (column, buffer, ...). The system automatically identifies the combinations of plasmids (if available) that can be used to produce any antibody.
Analysis of data from functional assays and quality control of purified antibody samples can be performed using the same tools used to do hit picking of library samples during the screening phase.
The full discovery and development history of an antibody is available and accessible by simple navigation throughout this network of relations. It can be used to answer questions like:
  • Has the VH-VL contained in this antibody been mutated, if so, how and from what?
  • Which other antibodies use this (or some variant of this) VH-VL?
  • Which productions and purifications have been made from this antibody (or any antibody containing this VH-VL)?